| Nanostructured Calcium Zirconium Silicate (Ca3ZrSi2O9) Bioceramic for Bone Tissue Engineering: An In Vitro/In Vivo Study |
| Paper ID : 1693-UFGNSM |
| Authors: |
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Razieh Kiani Harchegani1, Ali Doostmohammadi *2, Abdolreza Ardeshirylajimi1 1Shahrekord University 2Materials Department, Engineering Faculty, Shahrekord University |
| Abstract: |
| Over the past two decades calcium silicate (Ca-Si) based ceramics have been introduced as bioactive materials for bone tissue engineering applications and repairing bone defects. Recently, it’s reported that zirconium modified calcium-silicate-based (Ca3ZrSi2O9, Baghdadite) ceramics stimulate cell proliferation and osteogenic differentiation of osteoblasts. The aim of this work was preparation, characterization and evaluation of biological properties of Baghdadite in the nanoparticles form. These nanoparticles were synthesized by sol–gel method and were annealed at 1150 °C. Fabricated nanoparticles were investigated using XRD, FE-SEM and MTT in vitro, then implanted to the rabbit tibia as a bone animal model. The characterization results showed that nanoparticles with expected composition were successfully synthesized using sol-gel method. MTT result was also showed that these nanoparticles were not only non-toxic but also proliferation of bone marrow derived mesenchymal stem cells was increased 96 hours after cultured in the present of nanoparticle compared to the control. Histopathological results were also showed that site of defect almost completely regenerate when implanted by nanoparticles after six weeks. Taking together, according to the results, Ca3ZrSi2O9 nanoparticles as a bioceramic with excellent biological properties can be applied for improvement of bone lesions healing process in the orthopedic and dental application. |
| Keywords: |
| Baghdadite, Nano particle, Cytotoxicity, In Vitro/In Vivo evaluation |
| Status : Paper Accepted (Oral Presentation) |
