| Preparation of porous silicon oxide modified with APTES and application in drug delivery |
| Paper ID : 1655-UFGNSM-FULL |
| Authors: |
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Elham Rostami * Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran |
| Abstract: |
| Loading of levothyroxine into the 3-(Aminopropyl)triethoxysilane (APTES) nanostructured on porous silica (SiO2) films and its subsequent release behavior were studied in vitro. Nanostructured mesofilms are used to load and release the drug levothyroxine in vitro. A biocompatible and biodegradable form of mesoporous SiO2 is prepared by electrochemical etching of single crystalline Si, followed by thermal oxidation in air at 600°C, and then it has been modified by APTES. Loading of levothyroxine into APTES film showed that in addition to effects regarding the stability and release of the levothyroxine in 10 mM phosphate buffer solution in various pH (5.5, 6.5, 7.4) and human serum, surface properties will affect compounds affinity towards particles. In addition to the surface properties, the chemical nature of the levothyroxine and the loading solution seems to be critical for loading process. However, with poorly dissolving drugs, the loading into the mesoporous 3-(Aminopropyl)triethoxysilane (APTES) clearly improved dissolution. The morphology investigation of the surface roughness was characterized by AFM and HR-SEM and surface chemistry by Fourier-transform infrared spectroscopy. Amount of loaded drug determinated by Thermal Gravimetric Analysis (TGA). In vitro drug release profiles are characterized by an enzyme-linked immunosorbent assay (ELISA) and UV spectrophotometry in phosphate buffer solution (PBS) and human serum which confirms that the drug molecules are released in their active form. Release of levothyroxine was studied by ELISA, detecting in the three months. The concentration of levothyroxine increased and after a week, it was achieved to normal limitation (24 µg/ml). After nine weeks the concentration of levothyroxine was decreased. Finally during 8 weeks the initial dose between 12.5 to 24 µg/ml has been observed. |
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| Status : Paper Accepted (Poster Presentation) |
