| Magnetic graphene-based nanocomposite: An efficient anticancer delivery system |
| Paper ID : 1577-UFGNSM-FULL |
| Authors: |
|
abbas Jafarizad *1, mehdi jaymand2, Ali Taghizadehgh-Alehjougi3, saeed mohammadi nasr1, Amir Mohammad Jabbari1 1Faculty of Chemical Engineering, Sahand University of Technology, P.O. BOX: 51335-1996 Tabriz, Iran 2Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, P.O. BOX: 51656-65811, Tabriz, Iran 3Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, P.O. BOX: 25240 Erzurum, Turkey |
| Abstract: |
| The aim of this study is the development of an efficient anticancer drug delivery nanosystem using PEGylated graphene oxide/magnetite nanoparticles (PEG-GO/Fe3O4). The nanosystem was loaded with mitoxantrone (MTX) as a universal anticancer drug. The MTX-encapsulation efficiency (EE) and MTX-loading efficiency (LE) of the nanocomposite were evaluated through ultraviolet-visible (UV-vis) spectroscopy. The cytotoxicity effect of the MTX-loaded GO-PEG/Fe3O4 nanocomposite was studied against U87 MG cell line using MTT cell viablity assay. DNA fragmentation was used to evaluate the genotoxicity properties of the drug-loaded nanocomposite against U87 MG cells, and to detect the mechanism of action, the genes contributed in apoptosis (Casp 9, and Casp 3) and survival (BcL-2, BcL-xL, Survivin) have been investigated using quantitative real time-PCR. As the results of biological (MTT, DNA lader, and real time-PCR) assays, controlled drug release behavior of the developed nanosystem as well as the inherent physicochemical and biological characteristics of both magnetite nanoparticles and graphene nanomaterials, we envision that the GO-PEG/Fe3O4 nanocomposite may be applied as enhanced drug delivery system for various cancer therapies (e.g., brain cancer) using both chemo- and photothermal therapy methods. |
| Keywords: |
| Magnetite nanoparticles, Graphene oxide, Mitoxantrone, Controlled drug delivery, Cancer therapy |
| Status : Paper Accepted (Poster Presentation) |
