| Development of novel electrospun PCL/gelatin/alginate scaffold for vascular tissue engineering |
| Paper ID : 1339-UFGNSM-FULL |
| Authors: |
|
Mohammad Sadegh Nourbakhsh *1, Maria Ghasemi2 1Central Administration of Semnan University, Campus 1, Semnan, I. R. of Iran Zip Code: 35131-19111 2Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran |
| Abstract: |
| Tissue engineering is a promising solution for problem of organ or tissue shortage. Electrospinning is an inexpensive, effective, and simple method to produce non-woven nano-fibrous mats, which have intrinsically high surface to volume ratios, increased flexibility in surface functionalities, improved mechanical performances, and smaller pores than fibers produced using traditional methods. In this study, electrospun gelatin/polycaprolactone (PCL) and alginate scaffolds were successfully produced. The experimental design was conducted with Minitab software. Electrospinning was performed by two nozzles simultaneously which one containing PCL and another one containing gelatin and alginate solution. The scaffolds were analyzed using scanning electron microscopy, FTIR, XRD and contact angle measurement. SEM results showed that the average fiber diameter reached to 733 nm by optimization of electrospinning parameters that were suitable for the growth of endothelial cells. FTIR analysis confirmed the presence of alginate and gelatin in electrospun scaffolds. In X-ray diffraction, peak appeared in 2θ=24 which is the characteristic peak of PCL. With increase the volume fraction of gelatin and alginate polymers from 10% to 18% hydrophilicity of scaffold increased and contact angle decreased from 120° to 62°.Cell culture of HUVEC cells showed that the scaffolds support cell growth successfully. Proliferation and cell growth was observed at 7th day after cell seeding. The experimental design results showed that the optimal concentration for polymers for fabrication of an artificial blood vessel was 12% PCL, 15% gelatin and 3% alginate. |
| Keywords: |
| electrospinning, vascular issue engineering, poly caprolactone, gelatin, alginate, biocompatibility |
| Status : Paper Accepted (Poster Presentation) |
