| Biosynthesis of Highly Porous Bacterial Cellulose Nanofibers |
| Paper ID : 1195-UFGNSM-FULL (R1) |
| Authors: |
|
Hadi Hosseini *1, Mehrdad Kokabi2, seyyed mohammad mousavi3 1Islamic Republic of Iran, Tehran, Jalale Al Ahmad Highway, Tarbiat Modares University, 2Polymer Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box: 14115-114, Tehran, Islamic Republic of Iran 3Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-114, Tehran, Islamic Republic of Iran |
| Abstract: |
| Bacterial cellulose nanofibers (BCNFs) as a sustainable and biodegradable microbial polymer have drawn tremendous research attention in tissue engineering, bacterial sensors and drug delivery. BCNFs exhibit extraordinary properties such as high purity, high crystallinity, high water absorption capacity and excellent mechanical strength in the wet state. These awesome properties are attributed to BCNFs morphology. In this work, the bacterial strain, Gluconacetobacter xylinus (PTCC 1734, obtained from Iranian Research Organization for Science and Technology (IROST)), was used to produce BCNFs using bacterial fermentation in the culture medium containing: glucose, yeast extract and calcium carbonate under static condition at 29 °C for 10 days in an incubator. The biosynthesized BCNFs hydrogel was dried at ambient temperature and atmospheric pressure then characterized using Brunauer–Emmett–Teller (BET), the nitrogen adsorption isotherms (pore size and surface area), and Field emission scanning electron microscopy (FE-SEM) analysis. FESEM micrographs displayed highly interconnected and porous structure composed of entangled web-like continuous nanofibers with an average diameter of 48.5±2.1 nm. BET results depicted dried BCNFs at ambient temperature had IV isotherm type, according to the IUPAC classification, indicating a mesoporous product around 85% with a specific surface area (SBET) of 81.45 m2.g-1. These results are in accordance with the FESEM observations. |
| Keywords: |
| Bacterial Cellulose Nanofibers, Mesoporous, BET, Gluconacetobacter xylinus |
| Status : Paper Accepted (Poster Presentation) |
