| Synthesis, Characterization and Investigation Cytotoxicity of Cerium Oxide nanostructures on the Mesenchymal Stem Cell |
| Paper ID : 1007-UFGNSM-FULL |
| Authors: |
|
fatemeh sadat sangsefidi * Kashan University Ravalli Ghotb Blvd.
sangsefidi |
| Abstract: |
| In this study, CeO2 nanoparticles were synthesized via hydrothermal method using (NH4)2Ce(NO3)6 salt as a cerium source and the green capping agent. The results showed that the green capping agent has an important role in the shape and particles size. CeO2 nanoparticles were characterized via x-ray diffraction (XRD) pattern, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. The crystal structure of the CeO2 nanoparticles was studied by XRD pattern and the average size of crystallites calculated by Scherrer formula was increased from 5 nm to 20 nm at 600-700 ͦ C. We studied cytotoxicity effect of CeO2 nanoparticle on bone marrow mesenchymal stem cell. the cytotoxicity of the CeO2 nanoparticles was experimented using MTT assay. This method quantified number of viable and metabolically active cells in percentage. This method is on the principle of conversion of MTT dye into formazan crystals via cells with functional mitochondria. Cytotoxicity was measured after exposure of mesenchymal stem cells with 400, 200, 100, 50, 25 and 12.5 μM for 24h. The cytotoxicity of CeO2 nanoparticles on mesenchymal stem cells was investigated via MTT assay following 24h of exposure. The results showed that with increasing dosage levels of CeO2 nanoparticles reduced cell viability. |
| Keywords: |
| CeO2, Nanostructure, Hydrothermal, Cytotoxicity, Stem cell. |
| Status : Paper Accepted (Poster Presentation) |
